Biomedical Sciences Research Seminar

Elucidation of novel factors driving islet inflammation and pancreatic B-cell death in Type 2 Diabetes

Atomic structure of a biological molecule
Seminars

As part of the School of Science and Technology Biomedical Sciences Research Centre Seminar Series, Laura Lopez, NTU presents: Elucidation of novel factors driving islet inflammation and pancreatic B-cell death in Type 2 Diabetes.

  • From: Wednesday 31 October 2018, 1.10 pm
  • To: Wednesday 31 October 2018, 2 pm
  • Location: 282, Erasmus Darwin, Nottingham Trent University, Clifton Campus, Clifton Lane, Nottingham, NG11 8NS

Past event

Event details

As part of the School of Science and Technology Biomedical Sciences Research Centre Seminar Series, Laura Lopez, NTU presents: Elucidation of novel factors driving islet inflammation and pancreatic B-cell death in Type 2 Diabetes.

Abstract

Type 2 diabetes is a chronic metabolic disorder where failure to maintain normal glucose homeostasis is associated with, and exacerbated by, obesity and the concomitant elevated free fatty acid concentrations typically found in these patients. Hyperglycaemia and hyperlipidaemia together contribute to a decline in insulin-producing -cell mass through activation of pro-inflammatory signalling pathways. There are however a large number of molecules potentially able to modulate these pathways, and the mechanism(s) by which glucolipotoxicity induces inflammation remains poorly defined. Utilising Illumina HiSeq next generation sequencing technology we have analysed the -cell transcritptome to identify those genes and proteins most sensitive to glucose and fatty acid environment. Data shows that of those molecules potentially able to activate inflammatory pathways, the S100 family of proteins are amongst the most highly upregulated by glucose and fatty acids. Independent PCR and immunoblot analysis have further confirmed upregulation of the three most highly expressed family members, namely S100A3, A4, and A5. Importantly, my data has established a link between S100A4 and NF-κB activation that is driven by glucose and fatty acids. In order to determine wider mechanisms involved in the activation of NF-κB by S100A4, predictive pathway interaction maps have been generated for S100A4 based on the RNA-seq data. This approach has uncovered a potentially novel interaction with a pro-inflammatory transcription factor not previously associated with T2D. This provides a valuable strategy that can be further exploited to discover novel potential targets for therapeutic intervention in the treatment of type 2 diabetes.

This seminar is hosted by Dr Karin Garrie

All welcome.

For any enquiries please contact Dr John Dickenson

Location details

Room/Building:

282, Erasmus Darwin

Address:

Nottingham Trent University
Clifton Campus
Clifton Lane
Nottingham
NG11 8NS

Past event

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