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Jack C. Leo

Senior Lecturer

Department of Biosciences

Staff Group(s)
Academic Division Bioscience

Role

I am a member of the Antimicrobial Resistance, Omics & Microbiota group at NTU. I am module leader for BIOL40472: Medical Microbiology and I also teach in modules BIOL33211: Toxicology, BIOL33511: Environmental Forensic Assessment, BIOL45231: Molecular Microbiology, BIOL22371: Applied Microbiology, BIOL33391: Molecular Microbiology, BIOL10321: Introduction to Microbiology, and BIOL14406: Living Systems.

Career overview

I attained my PhD from the University of Helsinki in 2009. I then moved to Germany for a postdoctoral fellowship at the Max Planck Institute for Developmental Biology in Tuebingen, where I spent 3 years. Following this, I did a second postdoc at the University of Oslo, Norway, before becoming a group leader there. I joined NTU in June 2019.

Research areas

I study bacterial pathogenesis, focusing on autotransporters of Gram-negative bacteria. These proteins are self-contained secretion systems and surface molecules that mediate a number of virulence functions. The proteins are found widely in Gram-negative bacteria, and I have studied a variety of autotransporters from organisms such as Escherichia coli, Pseudomonas aeruginosa, and Yersinia enterocolitica. My work characterises both the functions of autotransporters as well as their secretion mechanism. In addition, as surface-exposed molecules and virulence factors, autotransporters are potential vaccine and drug targets.

Supervised PhD students:

Thomas Trunk (2016-2020, University of Oslo): Characterization and comparative analyses of type Vd-secreted phospholipases expressed in pathogenic bacteria. Defended on 18/6/2020.

External activity

I am a member of the editorial board for Biology (MDPI), PeerJ, and Scientific Reports, and I am a review editor for Frontiers in Microbiology. I also regularly review for journals such as FEMS Microbiology Letters, Toxins (MDPI), and Microbiology. For recent peer review activity, visit my Publons page.

I am a member of the Society for Applied Microbiology and the Microbiology Society.

Recent Conference Attendance:

8th FEMS Meeting, Glasgow, UK, 7.-11.2019. Oral presentation: Comparison of enzymatic activity and oligomer formation in type Vd-secreted phospholipases.

55th NBS Contact Meeting, Røros, Norway, 24.-27.1.2019. Oral presentation: Using the SpyCatcher-SpyTag technology for topology mapping of outer membrane proteins in Gram-negative bacteria.

Bacterial Protein Export 2018, Rega Institute, KU Leuven, Belgium, 30.9.-3.10.2018. Chairing a session on Sec secretome, biogenesis and protein folding (1.10.). Poster: Inverse autotransport: the mechanism of type Ve secretion.

16th Finnish Microbial Pathogenesis Days, Haartman Institute, University of Helsinki, Finland, 29.-30.8.2018. Oral presentation: Co-aggregation properties of trimeric autotransporter adhesins.

Gordon Research Conference: Bacterial Cell Surfaces, Mount Snow Resort, Vermont, USA, 24.-29.6.2018. Poster: Co-aggregation properties of trimeric autotransporter adhesins.

Biofilms 8 Meeting, Aarhus, Denmark 27.-29.5.2018. Poster presentation: Co-aggregation properties of trimeric autotransporter adhesins.

Recent Outreach Activities:

University of Oslo Open Day (Åpen dag), University of Oslo, Norway, 7.3.2019. Popular science presentation: Plastic-eating bacteria.

Sponsors and collaborators

I have a wide collaboration network with colleagues worldwide. Some of my recent collaboration partners are mentioned below:

Prof Katsutoshi Hori (University of Nagoya, Japan): atomic force measurments on bacterial adhesins.

Prof J. Preben Morth (Technical University of Denmark): structure and biophysics of bacterial adhesins.

Prof Dirk Linke (University of Oslo, Norway): adhesins from bacterial fish pathogens.

Dr Monika S. Schütz (University Clinics Tübingen, Germany): adhesins from human enteropathogens.

Prof Daniel J. Slade (Virginia Polytechnic and State University, USA): phospholipase autotransporters.

Research Funding:

2016 – 2019 Research Council of Norway Young Investigator Grant (“Type Vd secretion: a virulence factor of Pseudomonas aeruginosa?”, 7 000 000 NOK/ 3 years; funding for own position, a PhD student and consumables).

2014 - 2017 VISTA Postdoctoral Fellowship (“Formation mechanism of biological nanoparticles”, personal salary and 150 000 NOK/a research expenses)

2011 - 2013 FEMS Advanced Fellowship (“Bacterial Ig-like domain-containing adhesins”, personal fellowship, €25 000)

Publications

Recent publications:

  1. Khalil HS, Øgaard J, Leo JC (2020): Coaggregation properties of trimeric autotransporter adhesins. Microbiolyopen https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.1109 
  2. Whelan R, McVicker G, Leo JC (2020): Staying out or going in? The interplay of type 3 and type 5 secretion systems in adhesion and invasion of enterobacterial pathogens. Int J Mol Sci. 21(11): 4102. https://www.mdpi.com/1422-0067/21/11/4102
  3. Wrobel A, Saragliadis A, Pérez-Ortega J, Sittman C, Göttig S, Liskiewicz K, Spence MH, Schneider K, Leo JC, Arenas J, Linke D (2020): The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence. Environ Microbiol., 22(7): 2939-55. https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1462-2920.15051
  4. ElTahir Y, Al-Araimi A, Nair, RR, Autio, K, Tu H, Leo JC, Al-Marzooqi W, Johnson EH (2019): Binding of Brucella protein, Bp26, to select extracellular matrix molecules. BMC Mol Cell Biol., 20: 55. https://bmcmolcellbiol.biomedcentral.com/articles/10.1186/s12860-019-0239-7
  5. Trunk T, Casasanta MA, Yoo CC, Slade DJ, Leo JC (2019): Comparison of type 5d autotransporter phospholipase activities demonstrates a correlation between high activity and intracellular pathogenic lifestyle. Biochem J.,476(18): 2657-76.  https://portlandpress.com/biochemj/article-lookup/doi/10.1042/BCJ20190136
  6. Wrobel A, Leo JC, Linke D (2019): Overcoming fish defences: the virulence factors of Yersinia ruckeri. Genes 10(9): 700. https://www.mdpi.com/2073-4425/10/9/700
  7. Meuskens I, Saragliadis A, Leo JC, Linke D (2019): Type V secretion systems: An overview of passenger domain functions. Front Microbiol., 10: 1163. https://doi.org/10.3389/fmicb.2019.01163
  8. Hatlem D, Trunk T, Linke D, Leo JC (2019): Catching a SPY: using the SpyCatcher-SpyTag and related systems for labeling and localizing bacterial proteins. Int J Mol Sci., 20(9): 2129. https://doi.org/10.3390/ijms2009212
  9. Chauhan N, Hatlem D, Orwyck-Rydmark M, Schneider K, Floetenmeyer M, van Rossum B, Leo JC, Linke D (2019). Insights into the autotransport process of a trimeric autotransporter, Yersinia adhesin A (YadA). Mol Microbiol., 111(3): 844-62. https://doi-org.ezproxy.uio.no/10.1111/mmi.14195
  10. Klein K, Sonnabend M, Frank L, Leibiger K, Franz-Wachterl M, Macek  B, Trunk T, Leo JC, Autenrieth IB, Schütz M, Bohn E (2019). Deprivation of the periplasmic chaperone SurA reduces virulence and restores antibiotic susceptibility of multidrug-resistant Pseudomonas aeruginosa. Front Microbiol., 10:100. https://doi.org/10.3389/fmicb.2019.00100

See all of Jack Leo's publications...

Course(s) I teach on

  • Student using scientific equipment
    Postgraduate taught / Professional | Full-time / Part-time / Sandwich | 2020

    https://www.ntu.ac.uk/study-and-courses/courses/find-your-course/science-technology/pg/2020-21/biotechnology

  • Student using science lab
    Postgraduate taught / Professional | Full-time / Part-time | 2020

    https://www.ntu.ac.uk/study-and-courses/courses/find-your-course/science-technology/pg/2020-21/mres-molecular-microbiology

  • Student using science lab
    Postgraduate taught / Professional | Full-time / Part-time / Sandwich | 2020

    https://www.ntu.ac.uk/study-and-courses/courses/find-your-course/science-technology/pg/2020-21/molecular-microbiology