David Negus

David Negus

Lecturer/Senior Lecturer

School of Science & Technology

Role

Dr Negus is a lecturer and researcher in Microbiology at Nottingham Trent University. Dr Negus teaches on various microbiology modules at all degree stages. He supervises Masters and Undergraduate research projects based on the predatory bacterium Bdellovibrio bacteriovorus.

His research is focused on developing B. bacteriovorus as a “living antibiotic” and developing novel approaches to the treatment of antibiotic-resistant infections.

Career overview

Dr Negus received his BSc (Hons) in Microbiology from the University of Nottingham and obtained his PhD in the laboratory of Professor Peter Taylor at UCL (University College London) where he helped develop a novel treatment for anthrax infections.

Following this, he went on to complete a Postdoctoral position at the University of Nottingham with Professor Liz Sockett, investigating the predatory action of Bdellovibrio bacteriovorus in immune environments.

Research areas

Dr Negus is interested in developing the predatory bacterium B. bacteriovorus as a “living antibiotic” to treat drug-resistant infections. In particular, he is interested in the forced evolution and isolation of predators with enhanced properties in immune environments. He is also interested in the potential to use predatory bacteria in combination with anti-bacterial agents to treat polymicrobial infections.

Dr Negus has a broad interest in developing other novel therapeutics for the treatment of antibiotic-resistant bacteria, particularly therapies based on new modalities.

Sponsors and collaborators

  • Professor Liz Sockett (University of Nottingham)
  • Professor Peter Taylor (University College London)
  • Public Health England (Porton Down)

Publications

Negus D, Moore C, Baker M, Raghunathan D, Tyson H, Sockett RE. Predator versus Pathogen: How do predatory Bdellovibrio bacteriovorus interface with the challenges of killing Gram-negative pathogens in a host setting? Annual Review of Microbiology. 2017. Vol. 71:441-457.

Negus D*, Baker M*, Raghunathan D, Radford P, Moore C, Clark G, Diggle M, Tyson J, Twycross J, Sockett RE. Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer. Scientific Reports. 2017. DOI:10.1038/s41598-017-08060-4. *Joint first authors.

Stabler RA , Rosado H, Doyle R, Negus D, Carvil P, Kristjánsson J, Green D, Franco-Cendejas R, Davies C, Mogensen A, Scott J and Taylor PW. Impact of the Mk VI SkinSuit on skin microbiota of terrestrial volunteers and an International Space Station-bound astronaut. Microgravity. 2017. doi: 10.1038/s41526-017-0029-5.

McCarthy A, Negus D, Martin P, Pechincha C, Oswald E, Stabler RA, Taylor PW. Pathoadaptive Mutations of Escherichia coli K1 in Experimental Neonatal Systemic Infection. PLOS one. 2016. 11(11) e0166793.

Negus D, Vipond J, Hatch G, Rayner E and Taylor PW. Parenteral Administration of Capsule Depolymerase EnvD Prevents Lethal Inhalation Anthrax Infection. Antimicrob. Agents Chemother. 2015. 59(12): 7687-7692

Negus D and Taylor PW. A poly-γ-D-glutamic acid depolymerase that degrades the protective capsule of B. anthracis. Mol. Microbiol. 2014. 91(6):1136-47

Negus D and Taylor PW. Accelerated Storage Stability Testing of a Potential Anti-Anthrax Therapeutic, EnvD. Bio-protocol. 2014. 4(21): e1281. DOI: 10.21769/BioProtoc.1281

Negus D. and Taylor PW. Purification and Detection of a PDGA Depolymerase from Pusillimonas noertemannii. Bio-protocol. 2014. 4(21): e1280. DOI: 10.21769/BioProtoc.1280