graham pockley

Publications

Summary

Professor Pockley currently has > 220 publications. He has also co-edited a Special Issue entitled ‘Heat shock proteins as modulators and therapeutic targets of chronic disease: an integrated perspective’ (Edkins A.L., Price J.T., Pockley A.G., Blatch G.L. eds) for Philosophical Transactions of the Royal Society B, and books entitled ‘Molecular Chaperones and Cell Signalling’ (Henderson B., Pockley A.G. eds - Cambridge University Press),‘Prokaryotic and Eukaryotic Stress Proteins in Infectious Disease’ (Pockley A.G., Santoro M.S., Calderwood S.K. eds - Springer, New York) and ‘Cellular Trafficking of Molecular Chaperones in Health and Disease’ (Henderson B., Pockley A.G. eds - Springer, New York).

Selected Publications (2017 - present)

2024

Lindsay J.O., Hind D., Swaby L., Berntsson H., Bradburn M., Bannur, U., Byrne J., Clarke C., Desoysa L., Dickins B., Din S., Emsley R., Foulds G.A., Gribben J., Hawkey C., Irving P.M., Kazmi M., Lee E., Loban A., Lobo A., Mahida Y., Moran G., Papaioannou  D., Parkes M., Peniket A., Pockley A.G., Satsangi J., Subramanian S., Travis S., Turton E., Uttenthal B., Rutella S., Snowden J.A. (2024) A multicentre, randomised controlled trial to evaluate the efficacy of autologous haematopoietic stem cell transplantation with low dose cyclophosphamide mobilisation and reduced intensity conditioning, in refractory Crohn’s disease: the ASTIClite trial. The Lancet Gastroenterology and Hepatology, 9(4):333-345. doi: 10.1016/S2468-1253(23)00460-0.

Yazdi M., Hasanzadeh Kafshgari M., Khademi Moghadam F., Zarezade V., Oellinger R., Khosravi M., Haas S., Pockley A.G., Wagner E., Wollenberg B., Multhoff G., Bashiri Dezfouli A. (2024) Crosstalk between stimulated NK cell receptors and membrane Hsp70 on tumor cells: a combined computational and experimental study. Advanced Science, 11(14): 2305998. doi: 10.1002/advs.202305998.

Lindsay J.O., Hind D., Swaby L., Berntsson H., Bradburn M., Bannur C.U., Byrne J., Clarke C., Desoysa L., Din S., Emsley R., Foulds G.A., Gribben J., Hawkey C., Irving P.M., Johnson P., Kazmi M., Lee E., Loban A., Lobo A., Mahida Y., Moran G., Papaioannou D., Parkes M., Peniket A., Pockley A.G., Satsangi J., Subramanian S., Travis S., Turton E., Uttenthal B., Rutella S., Snowden J.A. (2024) Autologous stem cell transplantation with low-dose cyclophosphamide to improve mucosal healing in adults with refractory Crohn’s disease: the ASTIClite RCT. Efficacy and Mechanism Evaluation, 11(3). doi: 10.3310/CGLT7102

2023

Lennartz P., Thölke D., Bashiri Dezfouli A., Pilz M., Lobinger D., Messner V., Zanth H., Foulds G.A., Pockley A.G., Schmidt-Graf F., Multhoff G. (2023) Biomarkers in adult-type diffuse gliomas: Elevated levels of circulating vesicular Hsp70 serve as a biomarker in glioblastoma multiforme and increase NK cell frequencies in oligodendroglioma and astrocytoma. Biomedicines, 11, 3235. doi: 10.3390/biomedicines11123235.

Xanthopoulos A., Samt A.-K., Guder C., Taylor N., Roberts E., Herf H., Messner V., Anskar Trill, Holzmann K.L.K., Kiechle M., Seifert-Klauss V., Zschaeck S., Schatka I., Tauber R., Schmidt R., Enste K., Pockley A.G., Lobinger D., Multhoff G. (2023) Hsp70 – A universal biomarker for predicting therapeutic failure in human female cancers and a target for CTC isolation in advanced cancers. Biomedicines, 11, 2276. doi: 10.3390/biomedicines11082276.

Bashiri Dezfouli A., Stangl S., Foulds G.A., Lennartz P., Pilkington G.J., Pockley A.G., Multhoff G. (2023) Immunohistochemical, flow cytometric and ELISA-based analyses of intracellular, membrane-expressed and extracellular Hsp70 as cancer biomarkers. Methods in Molecular Biology, Stuart K. Calderwood S.K., Prince T.L. (eds.), Chaperones: Methods and Protocols, vol. 2693. doi: 10.1007/978-1-0716-3342-7_23.

Brentville V.A., Vankemmelbeke M., Methingham R.L., Symonds P., Cook K.W., Urbanowicz R.A., Tsoleridis T., Coleman C.M., Chang K-C., Skinner A., Dubinina E., Daniels I., Shah S., Argonza M., Delgado J., Dwivedi V., Kulkarni V., Dixon J.E., Pockley A.G., Adams S.E., Paston S.J., Daly J.M., Ball J.K., Durrant L.G. (2023) SARS-CoV-2 spike RBD and nucleocapsid encoding DNA vaccine elicits T cell and neutralizing antibody responses that cross react with variants. Journal of Biotechnology and Biomedicine, 6: 164-184. doi: 10.26502/jbb.2642-91280085.

Vaupel P., Piazena H., Notter M., Thomsen A.R., Grosu A-L., Scholkmann F., Pockley A.G., Multhoff G. (2023) From localized mild hyperthermia to improved tumor oxygenation: Physiological mechanisms critically involved in oncologic thermo-radio-immunotherapy. Cancers, 2023, 15(5), 1394. doi: 10.3390/cancers15051394.

2022

Dring K., Cooper S.B., Williams R., Morris J.G., Sunderland C., Foulds A., Pockley A.G., Nevill M.E. (2022) Effect of adiposity and physical fitness on cardiometabolic risk factors in adolescents: a 2-year longitudinal study. Frontiers in Sport and Active Living, Dec 15;4:1060530. doi: 10.3389/fspor.2022.1060530.

Seier S., Bashiri Dezfouli A., Lennartz P., Pockley A.G., Klein H., Multhoff G. (2022) Elevated levels of circulating Hsp70 and an increased prevalence of CD94⁺/CD69⁺ NK cells is predictive for advanced stage non small cell lung cancer. Cancers, Nov 21;14(22):5701. doi: 10.3390/cancers14225701.

Le Vu P., Vadakekolathu J., Idri S., Nicholls H., Cavaignac M., Reeder S., Khan M., Christensen D., Pockley A.G., McArdle S.E. (2022) A mutated Prostatic Acid Phosphatase (PAP)-peptide based vaccine induces PAP-specific CD8⁺ T cells with ex vivo cytotoxic capacities in HHDII/DR1 transgenic mice. Cancers, Apr 13;4(8):1970. doi: 10.3390/cancers14081970.

Howard F., El-Janabi H., Staniland S., Kennerley A., Patel P., Cox K., Allwood D., Smith E., Vadakekolathu J., Pockley A.G., Connor J., Collado-Rojas C., Muthana M. (2022) Bugs as drugs – Bacterial derived nanomagnets enhance tumour targeting and oncolytic activity of HSV-1 virus. Small 2022, e2104763. doi: 10.1002/smll.202104763

2021

Cosma G., McArdle S.E.B., Foulds G.A., Hood S.P., Reeder S., Johnson C., Khan M.A., Pockley A.G. (2021) Prostate Cancer: Early detection and assessing clinical risk using Deep Machine Learning of high dimensional peripheral blood flow cytometric phenotyping data. Frontiers in Immunology, 16 December 2021. doi: 10.3389/fimmu.2021.786828.

Cossarizza A., Chang H-D., Radbruch A., et al.  (2021) Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). European Journal of Immunology, 51: 2708–3145.

Bashiri Dezfouli A., Yazdi M., Pockley A.G., Khosravi M., Kobold S., Wagner E., Multhoff G. (2021) NK cells armed with chimeric antigen receptors (CAR): roadblocks to successful development. Cells, 10 (12). doi 10.3390/cells10123390:

McArdle S.E.M., Pawelec G., Pockley A.G., Johansen P. (2021) Editorial: Frontiers’ Research Topic ‘Cancer Vaccines: Time to Think Differently!’ Frontiers in Immunology, 12:771319. doi: 10.3389/fimmu.2021.771319

Tulotta C., Lefley D.V., Moore C.K., Amariutei A.E., Spicer-Hadlington A.R., Quayle L.A., Hughes R.O., Ahmed K., Cookson V., Evans C.A., Vadakekolathu J., Heath P., Francis S., Pinteaux E., Pockley A.G., Ottewell P.D. (2021) IL-1B drives opposing innate immune responses in primary tumours and bone metastases, limiting treatment options in breast cancer. npj Breast Cancer, 7:95. doi: 10.1038/s41523-021-00305-w

Brentville V.A., Vankemmelbeke M., Methingham R.L., Symonds P., Cook K.W., Urbanowicz R.A., Tsoleridis T., Coleman C.M., Chang K-C., Skinner A., Dubinina E., Daniels I., Shah S., Dixon J.E., Pockley A.G., Adams S.E., Paston S.J., Daly J.M., Ball J.K., Durrant L.G. (2021) A novel bivalent DNA vaccine encoding both spike protein receptor-binding domain and nucleocapsid protein of SARS-CoV-2 to elicit T cell and neutralising antibody responses that cross react with variants. bioRxiv, doi: 10.1101/2021.06.18.448932.

Almshayakhchi R., Nagarajan D., Vadakekolathu J., Reeder S., Brentville V., Metheringham R., Durrant L., Pockley A.G., McArdle S.E.B. (2021) A novel HAGE/WT1-ImmunoBody™ vaccine combination enhances anti-tumour responses when compared to either vaccine alone. Frontiers in Oncology,doi: 10.3389/fonc.2021.636977.

Nagarajan D., Pearson J., Brentville V., Metheringham R., Ahmad M., Durrant L., Pockley A.G., McArdle S.E.B. (2021) Helicase antigen (HAGE)-derived vaccines induce immunity to HAGE and ImmunoBody™-HAGE DNA vaccine delays the growth and metastasis of HAGE-expressing tumors in vivo. Immunology & Cell Biology, Jun 9.  doi: 10.1111/imcb.12485

Shackleton E.G., Ali H.Y., Khan M., Pockley A.G., McArdle S.E. (2021) Novel combinatorial approaches to tackle the immunosuppressive microenvironment of prostate cancer. Cancers, 13(5), 1145; doi: 10.3390/cancers13051145

Cuzzubbo S., Mangsbo S., Habra K., Nagarajan D., Pockley A.G., McArdle S.E.B. (2021) Cancer vaccines: Adjuvant potency, importance of age, lifestyle and treatments. Frontiers in Immunology, 17 Feb 2021. doi: https://doi.org/10.3389/fimmu.2020.615240

2020

Kwan A., Winder N., Atkinson E., Al-Janabi H., Allen R.J.,  Hughes R., Moamin M., Louie R., Evans D., Hutchinson M., Capper D., Cox K., Handley J., Wilshaw A., Kim T., Tazzyman S.J., Srivastava S., Ottewell P., Vadakekolathu J., Pockley A.G., Lewis C.E., Brown J.E., Danson S.J., Conner J., Muthana M. (2020) Macrophages mediate the anti-tumor effects of the oncolytic virus HSV1716 in mammary tumors. Molecular Cancer Therapeutics, 20 (3): 589-601 doi: 10.1158/1535-7163.MCT-20-0748.

Pearson J.R.D., Cuzzubbo S., McArthur S., Durrant L., Adhikaree J., Tinsley C.J., Pockley A.G., McArdle S.E.M. (2020) Immune escape in glioblastoma multiforme and the adaptation of immunotherapies for treatment. Frontiers in Immunology, 11:582106. doi: 10.3389/fimmu.2020.582106/full.

Multhoff G., Seier S., Stangl S., Sievert W., Shevtsov M., Werner C., Pockley A.G., Blankenstein C., Hildebrandt M., Offner R., Ahrens N., Kokowski K., Hautmann M., Rödel C., Fietkau R., Lubgan D., Huber R., Hautmann H., Duell T.,  Molls M., Specht H., Haller B., Devecka M., Sauter A., Combs S. (2020) Targeted natural killer cell based adoptive immunotherapy for the treatment of patients with NSCLC after radiochemotherapy – a randomized phase II clinical study. Clinical Cancer Research, 26:5368-5379. doi: 10.1158/1078-0432.CCR-20-1141.

Hood S.P., Cosma G., Foulds G.A., Johnson C., Reeder S., McArdle S.E., Khan M.A., Pockley A.G. (2020) Identifying prostate cancer and its clinical risk in asymptomatic men using Machine Learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data. eLife, e50936 doi: 10.7554/eLife.50936.

Abdel-Fatah T.M.A., Ball G.R., Thangavelu P.U., Reid L.E., McCart Reed A.E., Saunus J.M., Duijf P.H.G., Simpson P.T., Lakhani S.R., Pongor L., Győrffy B., Moseley P.M., Green A.R., Pockley A.G., Caldas C., Ellis I.O., Chan S.Y.T. (2020) Sperm associated antigen 5 (SPAG5) predicts and monitors response to endocrine and chemo-therapies in oestrogen receptor positive (ER+) breast cancer (BC): A potential tool for BC precision medicine. JAMA Network Open, 3(7):e209486, doi: 10.1001/jamanetworkopen.2020.9486.

Vadakekolathu J., Minden M.D., Hood T., Church S.E., Reeder S., Altmann H., Sullivan A.H., Viboch E.J., Patel T., Ibrahimova N., Warren S.E., Arruda A., Liang Y., Smith T.H., Foulds G.A., Bailey M.D., Gowen-MacDonald J., Muth J., Schmitz M., Cesano A., Pockley A.G., Valk P.J.M., Lowenberg B., Bornhauser M., Tasian S.K., Rettig M.P., Davidson-Moncada J., DiPersio J.F., Rutella S. (2020) Immune landscapes predict chemotherapy resistance and immunotherapy response in acute myeloid leukemia. Science Translational Medicine, 12: eaaz0463.

Gruet M., Cotton D., Coveney C., Boocock D.J., Wagner S., Komorowski L., Rees R.C., Pockley A.G., Garner A.C., Wallis J.D., Miles A.K., Powe D.G (2020) β2-adrenergic signalling promotes cell migration by upregulating expression of the metastasis-associated molecule LYPD3. Biology, 9(2), 39; doi: 0.3390/biology9020039.

Gaipl U.S., Multhoff G., Pockley A.G., Rödel F (2020) Editorial: Radioimmunotherapy – Translational Opportunities and Challenges. Frontiers in Oncology,  10:190. doi: 10.3389/fonc.2020.00190.

Nicklin M., Hickman G.J, Pockley A.G., Perry C.C. (2020) Materials-based approach for interrogating human prostate cancer cell adhesion and migratory potential using a Fluoroalkylsilica culture surface. ACS Applied Bio Materials, 3:495-504. doi: 10.1021/acsabm.9b00940

Pockley A.G, Vaupel P., Multhoff G (2020) NK cell-based therapeutics for lung cancer. Expert Opinion on Biological Therapy, 20(1):23-33. doi: 10.1080/14712598.2020.1688298.

2019

Cossarizza A., Chang H-D., Radbruch A. et al  (2019) Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 49: 1457-1973. doi: 10.1002/eji.201970107.

Sabry M., Zubiak A., Hood S.P., Arellano-Ballestro H., Simmonds P., Cournoyer E., Mashar M., Pockley A.G., Lowdell M.W. (2019) Tumor- and cytokine-primed human natural killer cells exhibit distinct phenotypic and transcriptional signatures. PLoS ONE, 14(6): e0218674, doi: 10.1371/journal.pone.0218674.

Snowden J.A., Hawkey C., Swaby L., Mellor K., Emsley R., Mandefield L., Lee E., Badoglio M., Polge E., Labopin M., Gribben J., Pockley A.G., Foulds G.A., Lobo S., Parkes M., Satsangi J., Papaioannou D., Lindsay J.O., on behalf of the Autologous Stem Cell Transplantation in Refractory CD Low Intensity Therapy Evaluation Study Investigators and the European Society for Blood and Bone Marrow Transplantation (EBMT) Autoimmune Diseases Working Party (ADWP) (2019) Autologous stem cell transplantation in refractory Crohn’s disease – low intensity therapy evaluation (ASTIClite): study protocols for a multicentre, randomised controlled trial and observational follow up study. BMC Gastroenterology 19:82, doi: 10.1186/s12876-019-0992-2.

Dring K.J., Cooper S.B., Morris J.G., Sunderland C., Foulds G.A., Pockley A.G., Nevill M.E. (2019) Multi-stage Fitness Test Performance, V̇O₂ peak & adiposity: effect on risk factors for cardio-metabolic disease in adolescents. Frontiers in Physiology, 10:629, doi: 10.3389/fphys.2019.00629.

Wagner S., Vadakekolathu J., Tasian S., Altmann H., Bornhäuser M., Pockley A.G., Ball G.R., Rutella S. (2019) A parsimonious 3-gene signature predicts clinical outcomes in an acute myeloid leukemia multi-cohort study. Blood Advances, 3: 1330-46, doi: 10.1182/bloodadvances.2018030726.

Hood S.P., Foulds G.A., Imrie H., Reeder S., McArdle S.E., Khan M.K. Pockley A.G. (2019) Phenotype and function of activated natural killer cells from patients with prostate cancer: Patient-dependent responses to priming and IL-2 activation. Frontiers in Immunology, 9:3169, doi: 10.3389/fimmu.2018.03169.

2018

Breuninger S., Stangl S., Werner C., Sievert W., Lobinger D., Foulds G.A., Wagner S., Pickhard A., Piontek G., Kokowski K., Pockley A.G., Multhoff G. (2018) Membrane Hsp70 - a novel target for the isolation of circulating tumor cells after epithelial-to-mesenchymal transition. Frontiers in Oncology, 8:497, doi: 10.3389/fonc.2018.00497.

Foulds G.A., Vadakekolathu J., Abdel-Fatah T.M.A. Nagarajan D., Reeder S., Johnson C., Hood S., Moseley P.M., Chan S.Y.T., Pockley A.G., Rutella S., McArdle S.E.M. (2018) Immune-phenotyping and transcriptomic profiling of peripheral blood mononuclear cells from patients with breast cancer: Identification of a 3 gene signature which predicts relapse of triple negative breast cancer. Frontiers in Immunology 9:2028, doi: 10.3389/fimmu.2018.02028.

Pockley A.G., Lindsay J.O., Foulds G.A., Rutella S., Gribben J.G., Alexander T., Snowden J.A. on behalf of the EBMT Autoimmune Diseases Working Party (ADWP) and the ASTIClite Study Investigators (2018) Immune reconstitution after autologous Haematopoietic Stem Cell Transplantation in Crohn's Disease: Current Status and Future Directions. A review on behalf of the EBMT Autoimmune Diseases Working Party and the ASTIClite Study Investigators. Frontiers in Immunology, 9:646. doi: 10.3389/fimmu.2018.00646.

Vadakekolathu J., Johnson C., Schneider A., Buczek M.E., Al-Juboori S., Pockley A.G., Ball G.R., Powe D.G., Regad T. (2018) MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer. Cell Death and Disease, 9(3):344. doi: 10.1038/s41419-018-0364-9.

Pockley A.G., Henderson B (2018) Extracellular cell stress (heat shock) proteins - immune responses and disease: An overview. Philosophical Transactions of the Royal Society B, 373(1738). pii: 20160522. doi: 10.1098/rstb.2016.0522.

Stangl S., Foulds G.A., Fellinger H., Pilkington G.J., Pockley A.G., Multhoff G. (2018) Immunohistochemical and flow cytometric analysis of intracellular and membrane-bound Hsp70, as a putative biomarker of glioblastoma multiforme, using the cmHsp70.1 monoclonal antibody. Methods in Molecular Biology, 1709:307-320. doi: 10.1007/978-1-4939-7477-1_22.

Edkins A.L., Price J.T., Pockley A.G., Blatch GL (2018) Heat shock proteins as modulators and therapeutic targets of chronic disease: an integrated perspective. Philosophical Transactions of the Royal Society B, 373(1738). pii: 20160521. doi: 10.1098/rstb.2016.0521.

2017

Abdel-Fatah T.M.A., Rees R.C., Pockley A.G., Moseley P., Green A.R., Ball G.R., Chan S.Y.T., Ellis I.O., Miles A.K. (2017) The localisation of pre‑mRNA splicing factor PRPF38B is a novel prognostic biomarker that may predict survival benefit of Trastuzumab in patients with breast cancer overexpressing HER2. Oncotarget, 8:112245-112257.

Cosma G., McArdle S.E., Reeder S., Foulds G., Hood S., Khan M., Pockley A.G. (2017) Identifying the presence of prostate cancer in individuals with PSA levels <20 ng/ml using computational data extraction analysis of high dimensional peripheral blood flow cytometric phenotyping data. Frontiers in Immunology, 8:1771. doi: 10.3389/fimmu.2017.01771.

Cossarizza A., Chang H-D., Radbruch A., et al (2017) Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, 47: 1584-1797.

Dunning-Foreman N., Vadakekolathu J., Laversin S.A., Morgan M.G., Reeder S., Pockley A.G., Rees R.C., Boocock D.J. (2017) A novel spontaneous model of epithelial-mesenchymal transition (EMT) using a primary prostate cancer derived cell line demonstrating distinct stem-like characteristics. Scientific Reports, 7:40633 | DOI: 10.1038/srep40633.

Selected Additional Key Publications

Abdel-Fatah T.M.A., Agarwal D., Liu D.-X., Russell R., Rueda O.M., Liu K., Moseley P.M., Green A.R., Pockley A.G., Rees R.C., Caldas C., Ellis I.O., Ball G.R., Chan S.Y. (2016) SPAG5 as a prognostic biomarker and chemotherapy sensitivity predictor in breast cancer: a retrospective, integrated genomic, transcriptomic, and protein analysis. Lancet Oncology, 17: 1004–8.

Cosma G., Acampora G., Brown D., Rees R.C., Khan M., Pockley A.G. (2016) Prediction of pathological stage in patients with prostate cancer: A Neuro-Fuzzy Model. PLoS ONE, 11(6): e0155856. doi:10.1371/journal.pone.0155856.

Abdel-Fatah T.M.A., McArdle S.E., Agarwal D., Moseley P.M., Green A.R., Ball G.R., Pockley A.G., Ellis I.O., Rees R.C., Chan S.Y.T. (2016) HAGE in Triple-Negative Breast Cancer is a novel prognostic, predictive, and actionable biomarker: A transcriptomic and protein expression analysis. Clinical Cancer Research, 22: 905-14.

Saif J.M.S., Vadakekolathu J., Rane S.S., McDonald D., Ahmad A., Mathieu M., Pockley A.G., Durrant L., Metheringham R., Rees R.C., McArdle S. (2014) A novel prostate acid phosphatase-based peptide vaccination strategy induces antigen-specific T-cell responses and limits tumour growth in mice. European Journal of Immunology, 44: 994-1004.

Gehrmann M., Stangl S., Foulds G.A., Oelinger R., Breuninger S., Rad R., Pockley A.G., Multhoff G. (2014) Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide. PLoS ONE 9(8): e105344. doi:10.1371/journal.pone.0105344.

Gehrmann M., Stangl S., Kirschner A., Foulds G.A., Sievert W., Doß B.T., Walch A., Pockley A.G., Multhoff G. (2012) Immunotherapeutic targeting of membrane Hsp70-expressing tumors using recombinant human granzyme B. PLoS ONE 7(7): e41341. doi:10.1371/journal.pone.0041341.

Stangl S., Gehrmann M., Riegger J., Kuhs K., Riederer I., Sievert W., Hube K., Mocikat R., Dressel R., Kremmer E., Pockley A.G., Friedrich L., Vigh L., Skerra A., Multhoff G. (2011) Targeting membrane heat shock protein 70 (Hsp70) on tumors by cmHsp70.1 antibody. Proceedings of the National Academy of Sciences USA, 108:733-8.

See a full list of Professor Pockley's publications on ORCID or Google Scholar