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Sarah Kuehne

Senior Lecturer

School of Science & Technology

Staff Group(s)
Bioscience

Role

Sarah Anne is a Senior Lecturer of Microbiology in the department of Biosciences. I am the head of the Antimicrobial Resistance, Omics and Microbiota (AROM: STAFF - AROM @ NTU (arom-ntu.net)) group research theme. My research focuses on understanding pathogenicity and lifestyle of anaerobic bacteria, bacterial communities (biofilms), including their communication and host-bacterial interactions.

She's a module lead for Clinical Public Health Microbiology (CPHM), BIOL22221: level 5 for Biomedical Science BSc (Hons) Undergraduate Course | Nottingham Trent University 

Microbiology BSc (Hons) Undergraduate Course | Nottingham Trent University

Sarah Anne is also Contributing to Research Methods and Bioethics (BIOL40102), postgraduate (MSc) module.

Career overview

2004 - 2008 PhD from the University of Nottingham working of posttranslational modification in Pseudomonas aeruginosa.

2008 – 2012 Postdoctoral Researcher in the Clostridial Research Group at the University of Nottingham.

2012 – 2016 Senior Research Fellow in the Clostridial Research Group at the University of Nottingham.

2016 – 2023 Assistant and since 2021 associated Professor of Microbiology at the University of Birmingham.

2023 – present Senior Lecturer in Microbiology at Nottingham Trent University.

Research areas

Research interests include the oral microbiome in health and disease, in particular the pathogenesis of Fusobacterium nucleatum and other anaerobes, multispecies biofilms, bacterial communication, novel antimicrobials and antimicrobial materials, as well as host-microbe interactions.

Ongoing projects:

  1. The role of Fusobacterium nucleatum in colorectal cancer.
  2. The role of the skin microbiome in Skin sensitivity – funded by the BBSRC and Boots
  3. Anaerobic biofilm fluidics – funded by the British Council in collaboration with BAM (BAM - Startseite) and Dr S. McLean (Samantha McLean | Nottingham Trent University)
  4. Communication in the oral cavity – funded by the BBSRC and Haleon
  5. Controlling Clostridioides difficile infections through modulation of gut bile acids – funded by NTU and the BRC-Nottingham
  6. Controlling Clostridioides difficile infections through phage therapy – funded by the BRC Nottingham in collaboration with Profs Nigel Minton and Klaus Winzer (Nigel Minton (sbrc-nottingham.ac.uk))

If you are interested to work in my group, then please get in touch. There will be availabilities for self-funded PhDs.

Further information may be obtained on the NTU Research Degrees (PhD and Professional Doctorates | Nottingham Trent University)

External activity

Memberships:

  • Member of the Microbiology Society
  • Member of the Prokaryote Division of the Microbiology Society (2016-2019)
  • Member of the Anaerobic Society of the Americas
  • Committee member (2016 – present)
  • Member of the Society for Anaerobic Microbiology (SAM)
  • Committee member (2022 – present)
  • Member of the Oral Microbiology and Immunology Group (OMIG)
  • Secretary of OMIG (2020 – present)

Editorial roles:

  • Section Editor: Journal of Medical Microbiology
  • Editor: Anaerobe
  • Reviewer for Anaerobe, Plos One, Microbial Genomics, Frontiers in Microbiology, Scientific reports and others

Publications

  1. Aly YM, Zhang Z, Ali N, Milward MR, Poologasundarampillai G, Dong H, Kuehne SA, Camilleri J. Ceramic conversion treated titanium implant abutments with gold for enhanced antimicrobial activity. Dent Mater. 2024 Aug;40(8):1199-1207. doi: 10.1016/j.dental.2024.05.029.
  2. Muguruza AR, Odyniec ML, Manhota M, Habib Z, Rurack K, Blair JMA, Kuehne SA, Walmsley AD, Pikramenou Z. Antibiotic entrapment in antibacterial micelles as a novel strategy for the delivery of challenging antibiotics from silica nanoparticles. Microporous and Mesoporous Materials 2024 Volume 363, 112841, doi.org/10.1016/j.micromeso.2023.112841.
  3. Bhandari SS, Palin W, Kuehne SA, Camilleri J. Investigating best practice for specimen preparation for biological testing of root canal sealers. Dent Mater. 2023 Dec 15:S0109-5641(23)00486-4. doi: 10.1016/j.dental.2023.12.001.
  4. Karatas E, Hadis M, Palin WM, Milward MR, Kuehne SA, Camilleri J. Minimally invasive management of vital teeth requiring root canal therapy. Sci Rep. 2023 Nov 21;13(1):20389. doi: 10.1038/s41598-023-47682-9.
  5. Nagi, M.; Chapple, I.L.C.; Sharma, P.; Kuehne, S.A.; Hirschfeld, J. Quorum Sensing in Oral Biofilms: Influence on Host Cells. Microorganisms 2023, 11, 1688. https://doi.org/10.3390/microorganisms11071688
  6. McCallum GE, Rossiter AE, Quraishi MN, Iqbal TH, Kuehne SA, van Schaik W. Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts. Microb Genom. 2023 Jun;9(6). doi: 10.1099/mgen.0.001030.
  7. Shirgill S, Poologasundarampillai G, Jabbari S, Ward J, Kuehne SA. Silver-doped bioactive glass fibres as a potential treatment for wound-associated bacterial biofilms. Biofilm. 2023 May 10;5:100115. doi: 10.1016/j.bioflm.2023.100115.
  8. Whittle MJ, Bilverstone TW, van Esveld RJ, Lücke AC, Lister MM, Kuehne SA, Minton NP. A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor. Microbiol Spectr. 2022 Feb 23;10(1):e0229521. doi: 10.1128/spectrum.02295-21.
  9. Simpson M, Bilverstone T, Leslie J, Donlan A, Uddin MJ, Petri WA, Marin N, Kuehne S, Minton NP, Petri WA Jr. Clostridioides difficile Binary Toxin Binding Component Increases Virulence in a Hamster Model. Open Forum Infect Dis. 2023 Jan 31;10(3):ofad040. doi: 10.1093/ofid/ofad040.
  10. Muchova M, Balacco DL, Grant MM, Chapple ILC, Kuehne SA, Hirschfeld J. Fusobacterium nucleatum Subspecies Differ in Biofilm Forming Ability in vitro. Front Oral Health. 2022 Mar 15;3:853618. doi: 10.3389/froh.2022.853618.
  11. Peritore-Galve FC, Shupe JA, Cave RJ, Childress KO, Washington MK, Kuehne SA, Lacy DB. Glucosyltransferase-dependent and independent effects of Clostridioides difficile toxins during infection. PLoS Pathog. 2022 Feb 17;18(2):e1010323. doi: 10.1371/journal.ppat.1010323.
  12. Jayash SN, Cooper PR, Shelton RM, Kuehne SA, Poologasundarampillai G. Novel Chitosan-Silica Hybrid Hydrogels for Cell Encapsulation and Drug Delivery. Int J Mol Sci. 2021 Nov 12;22(22):12267. doi: 10.3390/ijms222212267.
  13. Alexander J, Dong H, Bose D, Hassan AA, Soo SL, Zhang Z, Tao X, Kuehne S, Li X, Dong H. Novel Catalytic Ceramic Conversion Treatment of Ti6Al4V for Improved Tribological and Antibacterial Properties for Biomedical Applications. Materials (Basel). 2021 Nov 1;14(21):6554. doi: 10.3390/ma14216554.
  14. Monteford J, Bilverstone TW, Ingle P, Philip S, Kuehne SA, Minton NP. What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes. Anaerobe. 2021;102422. doi:10.1016/j.anaerobe.2021.102422
  15. Villapún VM, Balacco DL, Webber, MA, Hall T, Lowther M, Addison O, Kuehne SA, Grover LM, Cox SC. Repeated exposure of nosocomial pathogens to silver does not select for silver resistance but does impact ciprofloxacin susceptibility. Acta Biomater. 2021;S1742-7061(21)00494-3. doi:10.1016/j.actbio.2021.07.056
  16. Mountcastle SE, Vyas N, Villapun VM, Cox SC, Jabbari S, Sammons RL, Shelton RM, Walmsley AD, Kuehne SA. Biofilm viability checker: An open-source tool for automated biofilm viability analysis from confocal microscopy images. NPJ Biofilms Microbiomes. 2021 May 14;7(1):44. doi: 10.1038/s41522-021-00214-7.
  17. Mohamad SA, Milward MR, Hadis MA, Kuehne SA, Cooper PR. Photobiomodulation of mineralisation in mesenchymal stem cells. Photochem Photobiol Sci. 2021 May;20(5):699-714. doi: 10.1007/s43630-021-00047-5.
  18. Hall TJ, Hughes EAB, Sajjad H, Kuehne SA, Grant MM, Grover LM, Cox SC. Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold. Sci Rep. 2021 Feb 24;11(1):4491. doi: 10.1038/s41598-021-84060-9.
  19. Castro-Córdova P, Mora-Uribe P, Reyes-Ramírez R, Cofré-Araneda G, Orozco-Aguilar J, Brito-Silva C, Mendoza-León MJ, Kuehne SA, Minton NP, Pizarro-Guajardo M, Paredes-Sabja D. Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Nat Commun. 2021 Feb 18;12(1):1140. doi: 10.1038/s41467-021-21355-5.
  20. Mohamad SA, Milward MR, Kuehne SA, Hadis MA, Palin WM, Cooper PR. Potential for direct application of blue light for photo-disinfection of dentine. J Photochem Photobiol B. 2021 Feb;215:112123. doi: 10.1016/j.jphotobiol.2021.112123.
  21. Bilverstone TW, Garland M, Cave RJ, Kelly ML, Tholen M, Bouley DM, Kaye P, Minton NP, Bogyo M, Kuehne SA, Melnyk RA. The glucosyltransferase activity of C. difficile Toxin B is required for disease pathogenesis. PLos Pathog. 2020 Sep 22;16(9):e1008852. doi: 10.1371/journal.ppat.1008852.
  22. Mountcastle SE, Cox SC, Sammons RL, Jabbari S, Shelton RM, Kuehne SA. A review of co-culture models to study the oral microenvironment and disease. J Oral Microbiol. 2020 Jun 4;12(1):1773122. doi: 10.1080/20002297.2020.1773122.
  23. Vyas N, Sammons RL, Kuehne SA, Johansson C, Stenport V, Wang QX, Walmsley AD. The effect of standoff distance and surface roughness on biofilm disruption using cavitation. PLoS One. 2020 Jul 30;15(7):e0236428. doi: 10.1371/journal.pone.0236428.
  24. Kuehne SA, Cartman ST, Heap JT, Kelly ML, Cockayne A & Minton NP.  The role of toxin A and toxin B in Clostridium difficile infection. Nature 2010; 467: 711-713.

    25. For all my publications please visit: ‪Sarah Kuehne - ‪Google Scholar

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